| abstract |
The invention discloses a kind of expression vector containing dexamethasone inducible system, belong to plant fertility regulation and control field.Expression vector of the present invention contains the fusion of arabidopsis MS1 genes and mouse glucocorticoid receptor sterol binding domain genetic fragment composition, is the promoter of arabidopsis MS1 genes in the fusion upstream.The invention also discloses by the expression vector arabidopsis thaliana transformation and obtain can be recovered by induced by dexamethasone fertility transgenic arabidopsis method.The principle that the present invention can be combined dexamethasone and be induced using glucocorticoid receptor, acquisition can be recovered the recessive cytoblast sterile transgenic arabidopsis of fertility by induced by dexamethasone, in the case of genotype is not changed, the fertility for turning have the set system arabidopsis can freely be manipulated, it can be used to produce sterile line as maintainer, for hybrid seeding, replace artificial emasculation, save labour.The present invention is also the recessive cytoblast sterile of other crops using this system provides template. |