http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107043431-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 |
| filingDate | 2017-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107043431-B |
| titleOfInvention | Purification method of bacterial capsular polysaccharide |
| abstract | The invention discloses a purification method of bacterial capsular polysaccharide, which comprises the steps of treating a streptococcus pneumoniae fermentation culture solution by using an inactivating agent, adding acid to adjust the pH value to be less than or equal to 6.5, precipitating impurities, centrifuging and microfiltering to remove precipitates to obtain polysaccharide clarified liquid; performing ultrafiltration liquid exchange and concentration on the polysaccharide clarified solution by using a membrane with the molecular weight cutoff of 10-100 KDa, and performing chromatographic separation on the obtained capsular polysaccharide solution by using composite ion exchange chromatography and hydroxyl phosphate gray salt chromatography to obtain a purified capsular polysaccharide solution; concentrating and ultrafiltering capsular polysaccharide solution, and performing sterile filtration and storage. By combining the compound ion exchange chromatography and the hydroxyl phosphate gray salt chromatography, the protein and nucleic acid impurities can be effectively reduced to be lower than 1%, the quality of the purified polysaccharide product is higher than the standard requirement of European Union pharmacopoeia, and the recovery rate is more than 60%. |
| priorityDate | 2017-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.