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filingDate 2017-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_112d73ccff2365210f6eae440f2284e0
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publicationDate 2017-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107034209-A
titleOfInvention A kind of new method for building salt tolerant yeast bacterial strain
abstract The present invention relates to a kind of new method for building salt tolerant yeast bacterial strain, step is as follows:Using wild type T yeast as starting strain, WM2 is obtained;Build SPT15 gene mutations storehouse;Build SPT15 recombinant plasmid YEplac195 P T SPT15 storehouses;The recombinant plasmid storehouse built is imported into W303, dominant strain is chosen and extracts plasmid;The plasmid extracted is imported into WM2, that is, constructs new salt tolerant T yeast strains.This method expands the research range for transcription factor SPT15 mutation libraries, obtain a kind of new method for building salt tolerant yeast bacterial strain, the new salt tolerant T yeast strains gone out constructed by this method are used in sauce fermentation, after the content that amino-acid nitrogen is determined after fermenting-ripening, it was found that more amino-acid nitrogens can be produced during fermented sauce, verify that it has a clear superiority in soy sauce brewing.
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priorityDate 2017-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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