http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107022651-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2017-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107022651-B |
| titleOfInvention | Kit for rapidly detecting hepatitis C virus nucleic acid and detection method thereof |
| abstract | The invention discloses a kit for rapidly detecting Hepatitis C Virus (HCV) nucleic acid by a one-step method, which comprises a nucleic acid extracting solution, an RT-qPCR reaction solution, a negative control, a positive control and a positive reference substance. The invention mainly solves the defect that the existing kit in the market has long time for detecting nucleic acid. On the basis of the basic reaction principle of the conventional RT-qPCR, the invention realizes the denaturation of nucleic acid at lower temperature by changing the components of a reaction buffer solution and uniquely designing and synthesizing primers and probes, thereby shortening the time-denaturation and renaturation temperature difference of PCR and greatly shortening the whole reaction time as a result. The kit has higher detection sensitivity, specificity and repeatability, can detect six common genotypes of the hepatitis C virus, is also suitable for a conventional quantitative PCR instrument, and provides accurate basis for auxiliary diagnosis of hepatitis C virus infection and drug treatment monitoring of an infected person. |
| priorityDate | 2017-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 358.