http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107011433-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-795 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-795 |
| filingDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107011433-B |
| titleOfInvention | Extraction and purification method of nostoc sphaeroides biliprotein and purified phycocyanin |
| abstract | The invention discloses a purification method of nostoc sphaeroides blue protein, which is characterized by comprising the following steps: (1) selecting an anion exchange column to pass through the column; (2) preparing Tris-HCl Buffer solution Buffer A and Tris-HCl-NaCl Buffer solution Buffer B; (3) dissolving coarse powder of phycobiliprotein of nostoc sphaeroides with Buffer A, centrifuging, and filtering; (4) washing the pump, and balancing the ion exchange column by using Buffer A Buffer solution; (5) directly sampling the nostoc sphaeroides biliprotein dissolved in Buffer A prepared in the step (3), performing gradient elution by using Buffer B, and collecting eluent; (6) washing the ion exchange column with a saline solution, and then back-flushing with an alkaline solution; (7) ultrafiltering, concentrating, and dialyzing to obtain purified Nostoc sphaeroids Kutz blue protein water solution. The method is simple to operate, the concentration and the purity of the nostoc sphaeroides blue protein are obviously improved, and the method is suitable for industrial large-scale production. |
| priorityDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.