abstract |
The present invention aims to provide a monoclonal antibody useful for the specific determination of tartrate-resistant acid phosphatase 5b. Using the human recombinant tartrate-resistant acid phosphatase 5b (TRACP-5b) purified from silk glands of silkworm as an antigen, by cell fusion, it was obtained that the reactivity to TRACP-5b was higher than that of tartaric acid-resistant acid phosphatase 5a. (TRACP-5a) Reactivity-specific hybridomas of monoclonal antibodies against TRACP-5b. By using this monoclonal antibody, TRACP-5b in the test sample can be detected with high sensitivity and specificity. |