Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-319 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-11 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-106995813-B |
titleOfInvention |
Direct cloning of large genomic fragments and new technologies for DNA multi-molecule assembly |
abstract |
A new technology for direct cloning of large genomic fragments and DNA multi-molecule assembly. The present invention provides a method for homologous recombination, the method comprising treating two or more target nucleic acid molecules with a first exonuclease, and then allowing the treated target nucleic acid molecules to bind to a second exonuclease and an annealing protein. Homologous recombination is carried out in the presence of the target nucleic acid molecules in which homologous recombination occurs, sharing at least a sequence homology region. The invention also provides kits for use in the methods of the invention. |
priorityDate |
2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |