| abstract |
A new technology for direct cloning of large genome fragments and DNA multi-molecule assembly. The present invention provides a method for homologous recombination, the method comprising using a first exonuclease to treat two or more target nucleic acid molecules, and then allowing the treated target nucleic acid molecules to undergo the treatment in the second exonuclease and annealing protein Homologous recombination occurs in the presence of , wherein at least a region of sequence homology is shared between the target nucleic acid molecules that undergo homologous recombination. The invention also provides kits for use in the methods of the invention. |