| abstract |
The invention discloses terpene synthase for producing koraiol and application thereof, belonging to the field of synthetic biology. By providing a terpene synthase FGJ09920 which synthesizes koraiol, the nucleotide sequence of which is shown in SEQ ID NO: 2, together with genes related to the mevalonate pathway, a strain for producing koraiol was constructed. Of the mevalonate pathway from E.coli XL1-blue atoB Genes or idi The gene is over-expressed to synthesize a large amount of catalytic substrate farnesyl pyrophosphate FPP, which can further promote the production of koraiol. The terpene synthase provided by the invention has specificity and high efficiency, can improve the yield of koraiol, greatly overcomes the defects of large input amount of raw materials and low yield of koraiol, reduces the research cost, and ensures environmental protection. |