http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106979987-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-042 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2017-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106979987-B |
| titleOfInvention | The HPLC detection method of main active in Lanhuayao |
| abstract | The present invention relates to a kind of HPLC detection methods of main active in Lanhuayao, specifically includes the following steps: the preparation of (1) mixed reference substance solution: accurately weighing 3.61 mg of catechin, 0.83 mg of epicatechin, 0.35 mg of picroside Ⅰ and be placed in 10 mL volumetric flasks, after the dissolution of 5 mL methanol is added, mixed reference substance solution is made in constant volume；(2) preparation of sample solution: taking Lanhuayao sample, finely ground, mixes, accurate to claim 6.5123 g, is put into conical flask, 50 mL methanol are added in precision, and weighed weight is ultrasonically treated 1 h, lets cool, supply the weight of less loss, mix, be centrifuged 10 min, take supernatant liquor, filtration to get；(3) separation detection: chromatographic column: TC-C 18 ；Mobile phase: methanol: the elution of 0.5% acetate gradient, using gradient elution mode, flow velocity: 1.0 mL min ‑1 ；Detection wavelength: 278 nm；Column temperature: 25 DEG C；Sample volume: 10 μ L.The chromatography can be separated preferably, be measured, and chromatographic peak peak shape is good, and separating degree is higher. |
| priorityDate | 2017-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.