http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106967682-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2312 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2315 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2017-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106967682-B |
| titleOfInvention | NK cell in-vitro amplification method |
| abstract | The invention discloses an NK cell in-vitro amplification method which comprises the steps of (1) preparing a reagent A with a main component of 50ng/ml anti-CD 3 monoclonal antibody +50ng/ml anti-CD 16 monoclonal antibody, a reagent B with a main component of 300IU/ml I L-2 and a reagent C with a main component of 20ng/ml I L-12 +20ng/ml I L-15, preparing 40ml of lymphocyte separation liquid as a reagent D, (2) coating the reagent A in a T-175 culture flask, standing overnight at 4 ℃), treating peripheral blood to obtain cells and serum, (4) resuspending the cells, adding the reagent D and then centrifugally collecting the PBMC, (5) resuspending the PBMC in an X-VO VI 15 culture medium, adding the reagent B, the reagent C and autologous serum, incubating, and (6) supplementing the culture medium, the reagent B, the reagent C and the autologous serum on days 3, 6, 9, 12 respectively, collecting the cells on day 14, the benefit lies in that the anti-CD 3 and anti-CD 16 monoclonal antibody are inoculated in advance, and the purity of the cells is improved when the in-vitro culture is firstly. |
| priorityDate | 2017-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.