abstract |
The present invention relates to a kind of preparation method and application of high-purity polyphyllin Ⅵ, belong to Chinese herbal medicine and extract separation field.This method is concretely comprised the following steps:By Paris polyphylla or trilliaceae medicinal material coarse powder it is broken after, ethanol is extracted, extract solution carries out macroporous absorbent resin, silica gel and reversed material column chromatography successively, using ethyl acetate, second alcohol and water as eluant, eluent, sediment fraction is filtered after eluent recycling design, wash and dried, you can the polyphyllin Ⅵ of high-purity is obtained.The present invention has the advantages that operating procedure is easy to repeat, condition is easily controllable, amplification and solvent toxicity are small, easily reclaim, and the basic organic solvent-free of gained polyphyllin Ⅵ is remained, and purity is up to more than 98%.Pharmacodynamic study of the finished product polyphyllin Ⅵ through influences such as myocardial infarctions caused by rat myocardium from injury caused by mouse normobaric hypoxia tolerance, common carotid artery occlusion induced mice cerebrum ischemia, ischemia-reperfusion and isolated rat heart myocardial ischemia, effect is preferable, available for treatment cardiovascular and cerebrovascular disease product development. |