| abstract |
The invention discloses a kind of method that hybridized Chinese tuliptree body embryo generation is carried out using methyl jasmonate, including Seed sterilization, the induction of embryo callus, the foundation of embryogenic suspension cell line, the single celled acquisition of liquid and transition, the generation step of inducement crossbreeding Liriodendron body embryo, it is characterized in that, in the generation of inducement crossbreeding Liriodendron body embryo, addition methyl jasmonate and ABA, or individually add methyl jasmonate in the medium.The present invention's carries out the fast numerous method of hybridized Chinese tuliptree body using methyl jasmonate, in hybridized Chinese tuliptree Somatic Embryogenesis, add plant growth regulator MeJa and effectively improve body embryo inductivity, reduction abnormal rate, it is further to improve hybridized Chinese tuliptree body embryo luminous efficiency, with good practicality, beneficial to the foundation of hybridized Chinese tuliptree rapid propagation system. |