http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106957855-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-11012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
| filingDate | 2017-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106957855-B |
| titleOfInvention | Method for targeted knockout of rice dwarf gene SD1 by using CRISPR/Cas9 technology |
| abstract | The invention discloses a method for targeted knockout of rice dwarf gene SD1 by using CRISPR/Cas9 technology, which comprises the steps of determining a target site edited by a CRISPR/Cas9 system in a rice SD1 gene coding region according to the design principle of CRISPR/Cas9, designing a primer according to the sequence of the target site, constructing a CRISPR/Cas9 vector, transforming rice callus by using an agrobacterium-mediated method, and finally obtaining a dwarf rice strain containing no transgenic DNA fragment of SD1 mutation through screening identification. The method is applied to the breeding of the short-stalk rice variety, can avoid the work of hybridization breeding and greatly shortens the period of breeding the short-stalk rice variety. |
| priorityDate | 2017-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.