http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106957812-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0095 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y118-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-06 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-18 |
| filingDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106957812-B |
| titleOfInvention | Construction and application of cytochrome P450 enzyme and electron transfer system engineering bacteria thereof |
| abstract | The invention discloses construction and application of a genetic engineering strain for producing cytochrome P450 and an electron transfer system thereof, belonging to the technical field of biology. The strain is an engineering strain which uses endogenous ferredoxin and ferredoxin reductase of streptomyces griseus (ATCC13273) as a P450 enzyme CYP105D1 electron transfer system, can realize oxygen site demethylation specific to the L-tetrahydropalmatine, and provides feasibility for producing L-corydalmine by genetic engineering strain biotransformation. Meanwhile, the ferredoxin reductases capable of supporting CYP105D1 biocatalysis can improve the biotransformation capability of CYP105D1 to various natural products, can realize high-efficiency transformation on natural products such as warfarin and 7-ethoxycoumarin besides levo tetrahydropalmatine, and provide another example for high-efficiency biotransformation based on P450. |
| priorityDate | 2017-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 306.