http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106950326-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_217f2be73fd6be0be36dd55803d3bec5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8813 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1438747d553e31b18f0900b69e49149b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d081d083a74c6f3280e20678c580f6b |
| publicationDate | 2017-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106950326-A |
| titleOfInvention | The method of chemical labeling combination LC MS a kind of and its application in nucleotide analysis |
| abstract | The invention discloses the method for chemical labeling combination LC MS a kind of and its application in nucleotide analysis.The present invention uses N, N dimethyl-p-phenylenediamines（DMPA）Chemical labeling is carried out to 10 kinds of monophosphic acid nucleotides including the nucleotides of two kinds of modifications that methylate, while retention behavior of these modified nucleotides in reversed-phase liquid chromatography is significantly improved, their detection sensitivities in mass spectrum are also drastically increased.This method sensitivity is high, selectivity is high, excess marker reagent D MPA is effectively removed using liquid-liquid extraction, excessive activator EDC is removed using Strata X solid phase extraction columns, the cytidylic acid of the extremely low-abundance modification that methylates in detection organism can be quantified, can significantly improve separation of the nucleotides in LC and its in ESI MS detection sensitivities, the response of its signal is improved 12 orders of magnitude. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108693294-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108693294-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109917058-A |
| priorityDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.