http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106947805-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2017-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106947805-B |
| titleOfInvention | Fluorescent PCR kit and system based on septin9 gene methylation in ARMS-PCR method detection human peripheral dissociative DNA |
| abstract | The invention discloses a kind of fluorescence PCR method, kit and systems based on septin9 gene methylation in ARMS-PCR method detection human peripheral dissociative DNA.Method includes: that (a) carries out conversion process to the site CpG in the human peripheral dissociative DNA containing septin9 gene, so that the unmethylated site CpG is converted to UpG；(b) Fluorescence PCR is carried out using the human peripheral dissociative DNA after conversion process as template, it include: the specific primer and its probe sequence for detecting septin9 gene methylation, Taq enzyme, UNG enzyme, dNTPs, dUTP, Mg in fluorescent PCR system 2+ With PCR buffer, the first additive and Second addition, wherein the first additive includes bovine serum albumin(BSA), Second addition includes dimethyl sulfoxide, NH 4 Cl and aqueous sorbitol solution.Method of the invention can be avoided the false positive phenomenon that 1 site CpG methylation represents whole gene methylation level；Methylation level detection limit is down to 1%, high sensitivity. |
| priorityDate | 2017-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.