http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106939051-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K17-14 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-14 |
filingDate | 2017-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106939051-B |
titleOfInvention | Method for immobilizing protein in microchannel and application |
abstract | The present invention relates to a method for immobilizing a protein in a microchannel, comprising the steps of: step one, fusion recombination expression of the MagR and a target protein; step two, when the liquid containing the fusion recombinant protein is in the microchannel in the magnetic field, the target protein is immobilized on the inner wall of the microchannel; in the using process, the fused recombinant protein separated from the inner wall of the micro-channel is absorbed and recovered by a strong magnetic field at the outlet of the tail end of the micro-channel; step four, after the immobilized protein is used, the immobilized protein is removed by fluid flushing; and step five, after the immobilized protein is eliminated, repeating the step two to the step four, and realizing the repeated operation of 'immobilization-use-elimination-re-immobilization' of the protein in the microchannel. The invention also provides the application field of the method. The invention has the advantages that: the operation is simple and convenient, and the cost is low; is suitable for the inner surfaces of micro-channels made of different materials. |
priorityDate | 2017-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 532.