http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106932367-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2015-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106932367-B |
| titleOfInvention | It is a kind of to detect the method to interact between Rab albumen and its effector based on fluorescence resonance energy transfer method |
| abstract | The invention belongs to bioscience technology fields, and the method to interact between Rab albumen and its effector is specifically detected based on fluorescence resonance energy transfer method.Fluorescent analog mantGTP displacement and the protein bound GDP of Rab by natural GTP, the donor as fluorescence resonance energy transfer；The effector of Rab albumen and the carrier of green fluorescent protein GFP amalgamation and expression are constructed, fusion protein is used as the receptor of fluorescence resonance energy transfer by expression and purification later；It can use the directly detection Rab albumen of combination and the interaction between the effector of green fluorescent protein GFP amalgamation and expression in the form of mantGTP in vitro of method of the Fluorescence Spectrometer based on fluorescence resonance energy transfer.The present invention provides the new method of a kind of sensitive efficiently detection Rab albumen and the interaction of its effector, the perfect system of protein interaction. |
| priorityDate | 2015-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.