http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106929009-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1088 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-06 |
| filingDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106929009-B |
| titleOfInvention | A kind of polypeptide fluorescence probe and the preparation method and application thereof |
| abstract | The present invention relates to a kind of polypeptide fluorescence probes, polypeptide fluorescence probe be bonded as the amino of polypeptide with the isothiocyanic acid base of fluorescein isothiocynate obtained by, polypeptide is tetrapeptide DAPD or dipeptides, and any two kinds of amino acid is formed by dipeptides in these three amino acid of dipeptides D, A and P.Polypeptide fluorescence probe the preparation method comprises the following steps: fluorescein isothiocynate is added in the reaction vessel, then dissolved with methanol or water, polypeptide be added, makes the molar ratio 1.2-1.5:1 of fluorescein isothiocynate and polypeptide；Under the conditions of 20-40 DEG C of constant temperature, after being stirred to react 4-25h, rotary evaporation removes solvent and obtains crude product；Crude product obtains required pure products after high performance liquid chromatography separation, purifying and drying.The present invention is from bionical angle, the fluorescence probe prepared can rapidly and precisely identify sialic acid, to biochemistry, materialogy and medicine, more especially there is important theoretical and practical significance with treatment with the early diagnosis for the major disease that sialic acid is important marker. |
| priorityDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.