| abstract |
The invention discloses a kind of MTI-446 haptens, comlete antigen, antibody preparation method and application, including the artificial semiantigen of the anabasine pesticide MTI-446 with the furan structure of tetrahydrochysene 3, the preparation of comlete antigen and antibody and the foundation of corresponding detection method.It is low that detection method of the invention overcomes common photometry sensitivity, it is impossible to meets desired problem, establishes a kind of sensitivity high, low cost, the quick immunoassay method easily grasped.It is haptens structure with 1 methyl 2 amino 3 (furfuryl of tetrahydrochysene 3) guanidine, and coupling bovine serum albumin(BSA) is immunogene, and coupling ovalbumin is coating antigen.By immunogene by immune animal, antibody is purified out from antiserum, the MTI-446 immunoassay method set up using the antibody has the advantages that sensitivity is high, high specificity, and synthesis of semiantigen is easy, with preferable application prospect. |