http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106925241-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2220-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2220-42 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-281 |
| filingDate | 2017-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106925241-B |
| titleOfInvention | A method of fixed metal affinity material is prepared using pyridoxime 5-phosphate |
| abstract | The invention discloses a kind of methods and the application of the fixation metal affinity material in sample pre-treatments that chelating agent pyridoxime 5-phosphate is used to prepare to fixed metal affinity material.The present invention chooses several common nano material Y first and is chemically modified, and prepares amido modified nano material, obtains Y-NH 2 ；Pyridoxime 5-phosphate is connected to Y-NH again 2 On, obtain the Y-NH of surface modification phosphate radical 2 -PLP；The finally supported metal ion on phosphate radical, obtains Y-NH 2 ‑PLP‑M n+ .Method of modifying of the invention is simple, time saving, and will not destroy the shape characteristic of base material, and the modification carries out functionalization to base material, increases the practicability of base material.Y-NH 2 ‑PLP‑M n+ It can be directly as fiber material, sample pre-treatments for complex biological sample, in extraction process, pass through centrifugation or external magnetic field, achieve the purpose that material is separated with sample, has been applied successfully to reference polypeptide mixed liquor, milk enzymolysis liquid, human serum sample, the fields such as extraction of phosphorylated polypeptide in mouse brain lysate at present. |
| priorityDate | 2017-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.