Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-908 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-988 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90203 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-03004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
2017-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-106906227-B |
titleOfInvention |
Preparation method and application of soluble oxalate oxidase |
abstract |
The invention discloses a recombinant expression frame for efficiently expressing soluble oxalate decarboxylase, and also discloses a preparation method of oxalate oxidase capable of efficiently expressing solubility and activity in escherichia coli. Compared with the traditional method such as a commercially available kit for measuring oxalic acid and an HPLC method, the measuring method has the advantages of higher accuracy and sensitivity, short time consumption, low cost, easy high-throughput measurement of a large number of samples by an enzyme-labeling instrument and good practicability. The oxalate oxidase prepared by the invention can also be used for measuring the activity of other enzymes which take oxalate as a substrate or a product, and can also be used for measuring the content of oxalate in blood and urine of human and animals and various samples containing oxalate. |
priorityDate |
2017-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |