http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106906203-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_867fcbba33d52703b45456b126ce0dca |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2517-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0609 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K61-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K61-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075 |
| filingDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8eb9a1c03f5d9a9e860732520c3ff84e |
| publicationDate | 2017-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106906203-A |
| titleOfInvention | The gynogenesis abductive approach of imitative stichopus japonicus |
| abstract | The present invention relates to a kind of gynogenesis abductive approach of imitative stichopus japonicus, using electrostimulation, 2min is balanced during ovum first is moved into electro photoluminescence buffer solution before electro photoluminescence, mix up electric impulser parameter, 1ml ovums are drawn with suction pipe to be put into electric shock cup, you can carry out electro photoluminescence treatment；The electro photoluminescence buffer solution is ZimmermanShi buffer solutions, and specific composition is：0.1mmol/L Ca(C 2 H 3 O 2 ) 2 H 2 O, 1mmol/L K 2 HPO 4 , 0.5mmol/L Mg (C 2 H 3 O 2 ) 2 ·4H 2 O, 0.1mmol/L reduced glutathione, 1% BSA, 0.28mmol/L sucrose；The electric impulser parameter is field intensity 0.8kv/cm, the μ F of electric capacity 3,2 electric pulses.Ovum density during the electro photoluminescence is 11 × 10 4 Individual/ml.The method of the present invention is easy and effective, and after being induced through electro photoluminescence, the cleavage rates of imitative stichopus japonicus can reach more than 28%, and embryo survival can reach more than 98%. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110946100-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110946100-B |
| priorityDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.