http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106893757-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_867fcbba33d52703b45456b126ce0dca |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5011 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8eb9a1c03f5d9a9e860732520c3ff84e |
| publicationDate | 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106893757-A |
| titleOfInvention | The bioactivity SRB assay methods of meat sesame soft coral extract |
| abstract | The present invention relates to a kind of bioactivity SRB assay methods of meat sesame soft coral extract, step is as follows：(1) take the logarithm the tumour cell of phase, added per the μ L of hole 200 in 96 orifice plates, the μ L of sample solution 2 of various concentrations are added per hole；(2) 37oC cultures 17h；(3) centrifugation sucks supernatant；(4) the 80%TCA solution of 50 μ L precoolings is added per hole, is moved into and fix 1.5h in 4oC refrigerators, then with deionized water rinsing 5 times, room temperature is dried；(5) the μ L of 0.5%SRB dye liquors 50 are added per hole, after being stored at room temperature 30min, is rinsed 4 times with the 1%TCA aqueous solution of precooling, room temperature is dried；(6) dissolving of 150 μ L Tris solution and protein bound dyestuff are added per hole, is determined per hole absorbance OD values under 520nm with ELIASA.By formula IR (%)=(OD Blank -OD Sample )/OD Blank × 100% calculates cell proliferation inhibition rate (IR%).Assay method of the invention is simple and quick and testing result reliability, is suitable to popularization and application. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109524064-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109524064-A |
| priorityDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.