http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106892964-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_867fcbba33d52703b45456b126ce0dca |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 |
| filingDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8eb9a1c03f5d9a9e860732520c3ff84e |
| publicationDate | 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106892964-A |
| titleOfInvention | The preparation method of Ciona crude extract |
| abstract | The present invention relates to a kind of preparation method of Ciona crude extract, step is as follows：（1）Ciona is crushed and is homogenized；（2）By homogenate in 4 DEG C, 10000rpm centrifugation 20min, supernatant is collected；（3）Supernatant is heated to 70 DEG C, 30min is incubated；（4）Supernatant is centrifuged, rotating speed 10000rpm, time 20min, collects supernatant；（5）Supernatant adds the acetone solvent of three times volume, Ciona crude extract is turned into 70% acetone soln, is isolated and purified；（6）4 DEG C stand overnight；（7）Removal acetone supernatant, pelleting centrifugation, rotating speed 10000rpm, time 20min stay precipitation standby；（8）Precipitation is put into ventilating kitchen, removes acetone；（9）To be precipitated and dissolved in ultra-pure water, solution centrifugal, rotating speed 10000rpm, time 20min takes supernatant standby；（10）Supernatant carries out ultrafiltration retention with the filter membrane of 5KDa, remains larger than the active constituent of 5KDa.The method of the present invention can simply and rapidly extract the crude extract of Ciona, and the composition in crude extract is comprehensive and recovery rate is high. |
| priorityDate | 2015-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.