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filingDate 2015-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b34b36052227f2f88334d43f327d206
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publicationDate 2017-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106884006-A
titleOfInvention A kind of recombined adhenovirus and its construction method
abstract The invention belongs to biological technical field, specifically disclose a kind of recombined adhenovirus and its construction method, E 2 gene of Classical Swine Fever is inserted by CMV promoter multicloning sites downstream Bgl II and Sal the I restriction enzyme sites of adenovirus, reproductive and respiratory syndrome virus GP5 genes are inserted in CMV promoter downstream Kpn I and Not the I restriction enzyme sites of adenovirus.Recombined adhenovirus of the present invention can respectively express CSFV E 2 protein and reproductive and respiratory syndrome virus GP5 albumen in same adenovirus; pig after only can just can making to be immunized by one shot immunity is obtained to CSFV and the antibody of reproductive and respiratory syndrome virus simultaneously; reduce vaccine inoculation number of times and cost; the protecting effect ganged up against to CSFV and reproductive and respiratory syndrome virus is 87.5%; both solved the problems, such as that swine fever and blue-ear disease vaccine can not be immunized simultaneously, virulence returns strong and homologous recombination after also solving the problems, such as attenuated vaccine immunity.
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