| abstract |
The invention discloses a kind of people's umbilical cord MSC serum-free medium CS nano-granule freeze-dried powders of HA modification, it by mass percentage include following components: people's umbilical cord MSC serum-free medium activated protein ingredient 5~12%, chitosan 20~65%, sodium tripolyphosphate 1~15%, hyaluronic acid 20~65%, water≤5%.Preparation method is, first the stem cell serum-free culture supernatant with various active cell factor is coated using CS nanoparticle preparation process, and then be lyophilized, then make the active amino on CS nanoparticle surface that covalent coupling occur with the carboxyl on the surface HA and react, product of the present invention is made. |