http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106879467-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2017-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106879467-B |
| titleOfInvention | A kind of trembling poplar strain tissue culture method |
| abstract | The present invention provides a kind of trembling poplar strain tissue culture methods, remove 1~2mm of both ends of stem section, obtain explant the stem section with axillary bud after alcoholic solution immersion, aseptic water washing, disinfectant disinfection and sterile water rinse again successively；Explant is inoculated into adventitious bud differentiation and culture base, differentiation 30~45d of culture obtains trembling poplar adventitious bud；Trembling poplar adventitious bud, which is inoculated into subculture multiplication medium, carries out squamous subculture, obtains the trembling poplar adventitious bud of 1.2~2.0cm high；The trembling poplar adventitious bud of 1.2~2.0cm is transferred in adventitious bud rooting culture medium and carries out culture of rootage, obtains trembling poplar regeneration tissue-cultured seedling.Tissue culture method using the present invention, the survival rate for obtaining tissue-cultured seedling are 91.4~91.6%.The tissue culture method of the present invention can make, using a stem section as radix, can be proliferated to 10 000~100 000 plants of tissue-cultured seedling through cultivating in 1 year. |
| priorityDate | 2017-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.