http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106867970-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 |
filingDate | 2017-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106867970-B |
titleOfInvention | Hybridoma cell strain secreting anti-malachite green monoclonal antibody and application thereof |
abstract | The invention discloses a hybridoma cell strain secreting a malachite green resistant monoclonal antibody and application thereof, and belongs to the technical field of biology. The hybridoma cell strain is named as a hybridoma cell strain MG A7 with the preservation number of CCTCC NO: C201666. The preparation method comprises the steps of taking a conjugate formed by carboxyl recessive malachite green and bovine serum albumin as an antigen, immunizing a BALB/c mouse, fusing spleen cells of the immunized mouse with rejuvenated SP2/0 myeloma cells, performing cell culture by adopting a culture medium without antibiotics, and obtaining a hybridoma cell strain through multiple screening and cloning; the hybridoma cell strain secretes the IgM k-chain monoclonal antibody with high sensitivity and strong specificity, and can be used for rapid and accurate immunodetection and immunoassay of malachite green. |
priorityDate | 2017-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.