http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106867882-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-027 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502769 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-50851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
| filingDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106867882-B |
| titleOfInvention | Multi-chamber microfluidic PCR chip for aptamer screening and manufacturing method and application thereof |
| abstract | The invention discloses a multi-chamber microfluidic PCR chip for screening an adapter, which comprises a microfluidic PCR reaction plate, a temperature circulating heating plate, a glass substrate and a chip clamp, wherein a reaction liquid injection hole, a Z-shaped mixing unit, an inflow microfluidic channel, a PCR amplification chamber, an outflow microfluidic channel and a reaction liquid outlet hole are sequentially communicated on the PDMS reaction plate. The microfluidic PCR chip adopts a plurality of reaction chambers, so that the high quality, sufficient acquisition and nucleic acid variety diversity of a secondary library can be ensured; the microfluidic PCR chip can rapidly obtain the optimal amplification condition through a fluorescent real-time amplification curve, and amplify the nucleic acid sequence screened by the aptamer under the optimal condition. The invention also discloses a method for screening the aptamers by using the multi-chamber microfluidic PCR chip, which can be used for PCR amplification of screening of various target aptamers and provides good idea design and reference for other aptamer screening workers. |
| priorityDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.