http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106866825-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106866825-B |
| titleOfInvention | Silkworm internal reference protein GAPDH polyclonal antibody and preparation method thereof |
| abstract | The invention discloses a silkworm internal reference protein GAPDH polyclonal antibody and a preparation method thereof, wherein the preparation method comprises the following steps: firstly, constructing a prokaryotic expression vector GAPDH/pET-28 a: (1) extracting RNA of silkworms; (2) reverse transcription; (3) PCR amplification; (4) constructing GAPDH/pMD-19T; (5) constructing GAPDH/pET-28a recombinant plasmid; step two, prokaryotic expression of GAPDH fusion protein; step three, preparing a GAPDH polyclonal antibody; the prepared GAPDH polyclonal antibody has high specificity, low cost and short period, not only lays a foundation for further obtaining a commercialized GAPDH antibody, but also provides a powerful tool and a technical support for the function research of silkworm and other near-source insect proteins, and simultaneously makes up the defect that most of commercialized reference antibodies in the market are derived from mammals, and increases the diversity and the selectivity of the reference antibodies. |
| priorityDate | 2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 210.