http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106857498-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0284 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2016-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106857498-B |
| titleOfInvention | Cell cryopreservation protective solution without dimethyl sulfoxide and application thereof |
| abstract | The invention provides a cell cryopreservation protective solution without dimethyl sulfoxide, which comprises the components with the final concentration of 10-20% of bovine serum (v/v), 35-50% of sucrose (w/v) and 0.15-0.35mol/L of phosphate buffer solution, and the pH value is 7.0-7.4. The invention also provides a cell freezing method, which comprises the steps of centrifuging the cell suspension, then using the cell freezing protective solution to resuspend cell sediment, and when the cell concentration reaches 10 4 ‑10 7 And when the concentration is/ml, cooling the cells according to the adaptive cooling curve until the cells are stored in liquid nitrogen. The cell freezing protective solution can ensure that passage cells complete the cell freezing process under the condition of being separated from dimethyl sulfoxide, ensure the biological characteristics of the passage cells to be unchanged, simultaneously avoid the damage to the passage cells caused by the toxic action of the dimethyl sulfoxide, avoid the potential risk brought by the dimethyl sulfoxide in the process of preparing biological products by the cells, and has good application prospect. |
| priorityDate | 2016-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.