http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106857245-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106857245-B |
| titleOfInvention | A method of improving apple transgenic line regeneration efficiency |
| abstract | The invention discloses a kind of methods improving apple transgenic line regeneration efficiency, including：Roundleaf Malus spectabilis tissue-cultured seedling is cultivated one month in expanding breeding culture medium, and the callus of basal part of stem is cut with scalpel, is then transplanted to regenerated stem section in new culture medium；Regenerated stem section is cultivated one month in expanding breeding culture medium, crosscutting two to three knife of middle arteries of the functional leaf of full extension, Agrobacterium is taken to be transferred to after infecting in pre- differential medium, light culture is transferred to after 2-4 days in differential medium, it is cultivated under light after light culture 2 weeks, until differentiating resistant buds；Resistant buds are transplanted in subculture medium and are further cultivated 20-30 days；It selects plant height and reaches the regeneration plant of 1.5cm and be transplanted to root media, culture is transplanted for one month.Excised leaf regeneration efficiency of the present invention reaches 80.33%, expands numerous coefficient and reaches 8.2, rooting rate reaches 96.38%. |
| priorityDate | 2017-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.