| abstract |
The invention provides the polymorphic detection kit in the sites of CYP2C19 genes * 2, * 3, * 17, kit includes PCR buffer solutions, archaeal dna polymerase, LDR buffer solutions, DNA ligase, positive control, negative control.The present invention can directly enter performing PCR LDR reactions to the DNA for having extracted, and qualitative detection is carried out to the CYP2C19 genes in sample, and by reference gene sequence as internal reference, using UDG enzyme Pollution protections.Kit amplification method of the invention is simple, program is brief, easy to operate, Pollution protection, and testing result high specificity, susceptibility are high, result is apparent, with a high credibility, can be used for CYP2C19 genes qualitative detection in whole blood. |