http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834328-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y404-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-04 |
| filingDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106834328-B |
| titleOfInvention | S-ribosyl homocysteine lyase gene recombinant expression vector and expression method and application thereof |
| abstract | The invention discloses an S-ribosyl homocysteine lyase gene recombinant expression vector, an expression method and application thereof, wherein the gene recombinant expression vector is obtained by the following method: restriction enzymes Hind III and Xho I are used for synchronously digesting pET28-a vectors and LuxS gene purified products amplified by PCR respectively to obtain a luxS gene fragment with Hind III and Xho I cohesive ends and a pET28-a vector large fragment, agarose gel electrophoresis is adopted for respectively recovering the luxS gene fragment and the vector fragment, a ligation product is obtained by ligating the luxS gene fragment and the vector fragment, the ligation product is introduced into escherichia coli BL21 competent cells, and escherichia coli BL21 contains a gene recombination expression vector pET28 a-luxS. The protein obtained by the invention has great potential in the research and application fields of beneficial character development and harmful character control mediated by bacteria. |
| priorityDate | 2017-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 244.