http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834286-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2017-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106834286-B |
| titleOfInvention | Primer combination for one-step method rapid construction of amplicon library |
| abstract | The invention discloses a primer combination for rapidly constructing an amplicon library by one-step method, which comprises the following steps: an upstream fusion primer designed according to a target amplicon, wherein the upstream fusion primer comprises a first adaptor sequence and a specific upstream primer sequence designed according to the target amplicon; a downstream fusion primer designed according to the target amplicon, wherein the downstream fusion primer comprises a second adaptor sequence and a specific downstream primer sequence designed according to the target amplicon; an upstream universal primer comprising a third linker sequence, a barcode sequence, and a first linker sequence; and a downstream universal primer comprising a universal sequence and a second linker sequence. By applying the fusion primer combination, an amplicon library can be simply, conveniently and quickly constructed by 1 step, and because barcode is introduced before PCR initiation, the possibility of cross contamination between samples and libraries is greatly reduced. |
| priorityDate | 2017-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.