http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834208-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-066 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0602 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2016-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106834208-B |
| titleOfInvention | Ultrasonic disruption method of naked mole tissue in chromatin co-immunoprecipitation |
| abstract | The invention relates to the field of immunoprecipitation, in particular to an ultrasonic disruption method of naked mole tissue in chromatin co-immunoprecipitation, which comprises the steps of firstly adding a collagenase II solution, a hyaluronidase solution and a mixed solution of DPBS buffer solution in a volume ratio of 1:1:1 into the naked mole tissue, oscillating and digesting the tissue at 37 ℃, centrifuging to remove supernatant, and then carrying out the following conventional ultrasonic disruption steps. By using the method, the DNA-protein compound with proper fragment size can be better obtained, and a good foundation is laid for the success of the whole ChIP experiment later. |
| priorityDate | 2016-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.