| abstract |
The present invention provides a kind of compound and preparation method thereof for virus fluorescence label and living imaging.The structure of the compound is shown in formula I.The compound can be used for the fluorescent marker of polypeptide, albumen or virus.Compound shown in Formulas I can the cysteine to virus surface carry out specifical and efficient modification, fluorescent molecule is tagged on the cysteine of surface protein, can choose virus surface is without cysteine not influence its bioactivity site carry out mutation introduce cysteine, the compound shown in Formulas I is modified again, so that fluorescent molecule is tagged on cysteine.The present invention develops a kind of method that can carry out fluorescent marker to polypeptide, albumen and virus, and the label in 30 minutes to biomolecule may be implemented in this method, and efficiency does not influence the yield and infection ability of virus close to 100%. |