http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106811477-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2434 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
| filingDate | 2017-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106811477-B |
| titleOfInvention | A kind of method that the expression of glucosidase multicopy improves trichoderma reesei cellulase enzyme activity |
| abstract | The invention discloses a kind of glucosidase multicopies to express the method for improving trichoderma reesei cellulase enzyme activity, belongs to genetic engineering field.The method of the present invention includes the following steps: (1) building using promoter sequence-signal coding sequence-glucosidase coded sequence-terminator sequence as the glucosidase multiple copy expression cassette of basic unit.The signal coding sequence of each unit is all different in expression cassette.(2) restriction enzyme enzyme recognition site sequence is added at glucosidase multiple copy expression cassette both ends, expression cassette is building up on expression vector by restriction enzyme, DNA ligase.(3) recombinant expression carrier of building is transformed into trichoderma reesei, conversion has the Li's Trichoderma strains cellulose enzyme activity of recombinant vector to be improved.The present invention is combined using different signal peptides, avoids identical signal peptide from competing, to improve the enzyme activity of trichoderma reesei institute cellulase-producing. |
| priorityDate | 2017-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.