http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106801030-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-148 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2016-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106801030-B |
| titleOfInvention | Serum replacement composition suitable for in vitro culture of liver-like cells and use method thereof |
| abstract | The serum substitute composition suitable for in vitro culture of the liver-like cells contains 4 vitamins, 2 amino acids, 2 cytokines, 3 proteins, 3 metal ions, 4 hormones, 2 fatty acids, 4 antioxidants and other substances; the use method of the serum replacement composition comprises the following steps: preparing a serum-free medium containing a substitute serum composition suitable for in vitro culture of liver-like cells; preparing a culture medium; the use of a serum-free medium containing a serum replacement composition suitable for in vitro culture of liver-like cells; fourth, passage or harvest of the liver-like cells cultured in vitro. The invention provides a substitute serum composition which has definite components and can be added into DMEM/F12 basal medium to be suitable for in vitro culture of liver-like cells; the using method has simple and clear operation steps, and is beneficial to the in-vitro serum-free culture and amplification of the liver-like cells; can provide positive support for the application of the liver-like cells in drug research and clinical treatment, and has good social and economic benefits. |
| priorityDate | 2016-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 390.