| abstract |
The invention belongs to Biological Detection technical field, and in particular to a kind of HIV antigen and antibodies card and preparation method thereof.Detection card includes Ka Gai, card body and test strips, and the card covers and is sequentially provided with dilution well, sample application hole, peephole and installs sign hole, and the inside of card body is provided with the groove for placing test strips；The test strips are sequentially provided with sample bed course, colloid layer gold, nitrocellulose filter and water accepting layer.Its preparation method includes：Preparation, the collaurum of collaurum mark the antigens of Recombinant HIV 1/HIV 2 and the anti-p24 monoclonal antibodies of mouse, biotin labeling p24 antibody, collaurum and biotinylated derivative immobilization, coating nitrocellulose filter, the preparation of test strips, the fastening of detection card respectively.Using the detectable HIV antigen-antibodies of detection card of the invention, sensitivity is high, and sample consumption is few. |