http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106755602-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2017-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106755602-B |
| titleOfInvention | Method for synchronously detecting SPVG, SPLV and SPMMV |
| abstract | The invention relates to a method for synchronously detecting SPVG, SPLV and SPMMV, belonging to the field of plant protection; a real-time fluorescent multiplex RT-PCR method for simultaneously detecting three viruses is established by respectively designing and synthesizing forward primers, reverse primers and probes of SPVG, SPLV and SPMMV, and the primers and the probes for detecting plant cytochrome oxidase genes (COX) are used as internal parameters to be built in a real-time fluorescent multiplex RT-PCR system, so that false negative results caused by poor template nucleic acid quality are avoided. The detection sensitivity of the invention to SPVG reaches 10 ‑6 The nucleic acid template has dilution multiple, and the detection sensitivity to SPLV, SPMMV and COX respectively reaches 10 ‑7 The dilution multiple of the nucleic acid template has the advantages of intuition, rapidness, high efficiency, strong specificity, high sensitivity and the like, can ensure the quality and efficiency of SPVG, SPLV and SPMMV in the detection of a large number of samples such as plant quarantine, sweet potato non-toxic seedling screening and the like, and has wide application prospect. |
| priorityDate | 2017-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.