http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106755456-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2017-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106755456-B |
| titleOfInvention | Primer combination and kit for mitochondrial whole genome detection |
| abstract | The invention relates to a primer combination and a kit for detecting a mitochondrial whole genome, which detect the mitochondrial whole genome by utilizing PCR and Sanger sequencing technologies to assist in diagnosing mitochondrial diseases caused by mtDNA mutation. The invention firstly utilizes 6 pairs of specific primers to amplify the whole mitochondrial genome, the size of each amplification product is 2700-3500bp, the adjacent amplification fragments have the overlap of more than 200bp, and then utilizes 21 sequencing primers to directly sequence the amplification products, and the sequencing range covers the whole mitochondrial genome. By utilizing the primer combination, the whole mitochondrial genome can be detected only by 6 amplification reactions and 21 sequencing reactions, so that the detection workload is greatly reduced, and the detection cost is also greatly reduced. |
| priorityDate | 2017-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.