http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106755059-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
| filingDate | 2016-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106755059-B |
| titleOfInvention | Backbone plasmid vector for genetic engineering and application |
| abstract | The invention discloses a backbone plasmid vector for genetic engineering, which comprises a crRNA expression frame and a Cpf1 nuclease expression frame, wherein the crRNA expression frame and the Cpf1 nuclease expression frame are positioned in the same binary vector, and the crRNA expression frame is sequentially composed of a rice U3 promoter, a spectinomycin resistance gene and a Poly-T terminator; the LbCpf1 nuclease expression frame consists of a ZmUBI promoter, an LbCpf1 coding sequence modified by rice preferred codons and a 35s terminator in sequence. The invention also provides a recombinant vector containing a target sequence constructed by using the plasmid vector and application thereof in rice gene targeting. The backbone plasmid vector can be used for effectively targeting genes of target fragments to obtain rice plants with random insertion and/or random deletion on the target fragments in the target genes. |
| priorityDate | 2016-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.