http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106755053-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
| filingDate | 2017-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106755053-B |
| titleOfInvention | A kind of double gene interference improves the method of Aspergillus niger cellulase enzyme activity |
| abstract | The invention discloses a method for improving cellulase activity of Aspergillus niger by double-gene interference, and belongs to the technical field of genetic engineering. The method of the present invention is to reduce the expression of Aspergillus niger phytase gene and glucosidase gene by RNAi, specifically comprising the following steps: the two ends have restriction endonuclease recognition sites and contain the interference of the sequence shown in SEQ ID NO.1 The fragment is constructed on pSilent-1 by corresponding restriction endonuclease and DNA ligase; the constructed recombinant vector is transformed into Aspergillus niger, and the enzyme activity of Aspergillus niger cellulase transformed with the recombinant vector is improved. Through the rational design of RNAi interference sequence, the present invention not only significantly reduces the expression of phytase, but also moderately reduces the expression of glucosidase, enhances the expression of exocellulase, and optimizes the activity ratio of the three enzymes in the cellulase enzyme system , Increased Aspergillus niger cellulase activity. |
| priorityDate | 2017-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.