http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754878-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754878-B |
| titleOfInvention | Method for constructing breast cancer susceptibility gene variation library |
| abstract | The invention relates to a method for constructing a breast cancer susceptibility gene variation library, which comprises the following steps: A. designing a primer pair capable of detecting the variation regions according to the variation regions of the breast cancer related genes; B. calculating to obtain a judgment parameter R of each primer pair, classifying the primer pairs with the judgment parameter R value less than or equal to 1 as a first group of primer combination liquid, and classifying the primer pairs with the judgment parameter R value greater than 1 as a second group of primer combination liquid; C. extracting and purifying DNA of a sample to be detected; D. respectively adopting a first group of primer combination solution and a second group of primer combination solution to carry out initial PCR amplification on the purified sample; E. performing joint connection on the target fragments to obtain fragments with joints; F. and performing PCR amplification on the library by adopting a mixed solution of the first group of primer combination solution and the second group of primer combination solution to obtain a sequencing library. The library constructed by the method for constructing the breast cancer susceptibility gene variation library has high sequencing flux, strong sensitivity and strong specificity, and can be used for detecting free DNA low-frequency mutation. |
| priorityDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 172.