http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754850-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01015 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 |
| filingDate | 2017-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754850-B |
| titleOfInvention | The glutamic acid decarboxylase enzyme mutant and its application that thermal stability improves |
| abstract | The glutamic acid decarboxylase enzyme mutant improved the present invention relates to thermal stability and its application, belong to bioengineering field.Mutant of the present invention is mainly that 5-7 glutamine Q, the valine V and threonine T of glutamic acid decarboxylase enzyme amino acid sequence are carried out saturation mutation, and screening obtains high stable mutant Q5E/V6S/T7V, Q5Y/V6R/T7K, Q5N/V6Y/T7V.Half deactivation temperature of the glutamic acid decarboxylase enzyme mutant that the present invention obtains is 45-50.5 DEG C, improves 4.9-10.2 DEG C than wild type；Half-life period at 45 DEG C is 76-129min, improves 3.2-4.3 times than wild type (24min).With the glutamic acid decarboxylase enzyme mutant resting cell glutamic acid 12h that recombination bacillus coli synthesizes, the γ-aminobutyric acid (GABA) of 260-350g/L, molar yield 76.6%-97.8% can be obtained.The obtained glutamic acid decarboxylase enzyme mutant thermal stability of the present invention significantly improves, more conducively the production requirement of γ-aminobutyric acid, lays a good foundation to efficiently synthesize γ-aminobutyric acid. |
| priorityDate | 2017-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 343.