http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754840-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-23004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate | 2016-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754840-B |
| titleOfInvention | The curdled milk enzyme mutant that a kind of enzyme activity and thermal stability improve |
| abstract | The invention discloses the curdled milk enzyme mutants that a kind of enzyme activity and thermal stability improve, and belong to technical field of enzyme engineering.The present invention replaces the 75th leucine of renin with glutamine, the 222nd methionine is replaced with threonine, and double mutation building curdled milk enzyme mutants are carried out to the amino acid in above-mentioned site, by mutant in Pichia pastoris GS115 heterogenous expression, curdled milk enzyme activity is carried out to the crude enzyme liquid of expression, protease activity, heat resistance detection.The crude enzyme liquid curdled milk enzyme activity of double-mutant Leu75Gln-Met222Thr reaches 28.0SU/mL, and C/P value reaches 11.7, and the curdled milk enzyme activity and C/P value compared to starting strain P-cMCE improve 155.6% and 195.0%.The thermal stability of 45 DEG C of constant temperature half an hour reduces 13.6%. |
| priorityDate | 2016-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 229.