http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106754647-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0625 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2017-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106754647-B |
| titleOfInvention | A kind of separation, culture and the identification method of giant salamander skin epidermal cells |
| abstract | The invention discloses a kind of separation, culture and the identification method of giant salamander skin epidermal cells, this method is to obtain skin histology from giant salamander tail portion, and primary giant salamander skin epidermal cells are obtained using tissue block method's separation, at 28 DEG C and specific culture solution (ingredient：DMEM/F12 (60%)+15%FBS+5 μ g/mL insulin+10ng/mL KGF) the primary and secondary culture of middle progress, and specific gene (K5, K10 and P63) expression identification is carried out to the giant salamander skin epidermal cells of acquisition using RT PCR.The present invention carries out PCR amplification using the homology design primer of close species gene sequence to giant salamander K5, K10 and P63 gene, obtains giant salamander K5, K10 and P63 gene order for the first time.The method of separation, culture and the identification of giant salamander skin epidermal cells provided by the invention carries out skin regeneration, wound repair and builds organization engineering skin to lay a good foundation for the deep structure research of giant salamander skin and using giant salamander skin epidermal cells. |
| priorityDate | 2017-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 166.